Cytotoxicity extract and fraction of knobweed (Hyptis capitata) and its effect on migration and apoptosis of T47D cells

Abstract. To’bungan N, Pratiwi R, Widyarini S, Nugroho LH. 2021. Cytotoxicity extract and fraction of knobweed (Hyptis capitata) its effect on migration apoptosis T47D cells. Biodiversitas 23: 572-580. Hyptis capitata Jacq. has an ethnobotanical history. The leaves stems H. have been reported to potential anticancer properties. This study was conducted determine the roots, stems, leaves, flowers capitata. Organs were extracted in stages using chloroform continued with methanol obtain eight extracts. Crude extracts tested for cytotoxicity WiDr cells MTT method. Potential inhibition cell by scratch wound healing assay content secondary metabolites analyzed gas chromatography-mass spectrometry (GC-MS). An Annexin V-Pl test performed number living cells, necrosis, apoptosis. results showed that root highest cytotoxic activity against (34.90 ± 4.78µg/mL) (44.65 12.07 µg/mL), it referred as a extract. Fractionation resulted four fractions (F1, F2, F3, F4). Fraction F2 had (13.8 0.65 µg/mL) selective (selectivity index 3.71). antimetastatic apoptotic Gas analyses combined library contain ferruginol, campesterol, stigmasterol.